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CABRI:ICLC |
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Accession_number |
ICLC HTL01017
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Cell_line_name |
5637
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Brief_description |
Species: human, Caucasian; Tissue: bladder; Tumor: carcinoma
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Description |
Species: human, Caucasian male 68 year old; Tissue: bladder; Tumor: carcinoma
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Depositor |
Dr M. Musso, Oncology, Biology and Genetic Dept, Genoa, Italy
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Bibliographic_reference |
Natl Cancer Inst Monogr 1978;49:5-9 [PMID: 571047
] |
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Morphology |
epithelial, grown as monolayer
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Culture_conditions |
continuous culture; RPMI 1640 + 10% FBS + 2mM L-Glutamine; split confluent cultures 1:4-1:8 using trypsin/EDTA (8-10 min.); 37C, 5% CO2
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Viruses |
Search for viruses was not performed
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Properties |
GM-CSF, IL-1, IL-6 production
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Release_conditions |
Cell line available for distribution. For non-commercial investigative use only
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Hazard |
-
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Species_validation |
Validated by HLA-DP beta: confirmed as human by PCR
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Tumorigenicity |
tumorigenic in nude mice
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Freezing_medium |
Culture medium + 50% FBS + 10% DMSO
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Sterility |
mycoplasma contamination eliminated with MRA; negative, HOECHST and PCR
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Validation_assays |
DNA Profile STR (Short Tandem Repeat): Amelogenin: X,Y; vWA: 18; FGA: 22; TH01: 7,9; D18S51: --; D21S11: 36; D8S1179: 10,16
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Further_bibliography |
J Natl Cancer Inst 1977;59:221-226 [PMID: 327080
]
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